A worldwide e-Infrastructure for NMR and structural biology

This site uses cookies for a better experience. By continuing to browse you agree to the use of cookies.

Homology Modeling and Structural Analysis of NHX Antiporter of Leptochloa fusca (L.)

AuthorsBahman Panahi1*, Nasrin Moshtaghi1, Ibrahim Torktaz2, Askar panahi3 and Sudeep Roy4

Eukaryotic NHX transporters are trans-membrane proteins which are vital for cellular homeostasis. Plants
vacuolar NHX antiporters have been suggested to be involved in sequestering Na+ into vacuoles, thus preventing
toxic effects of Na+ in the cytoplasm. Primary and secondary structure analysis of NHX antiporter of Leptochloa
fusca suggested that this antiporter is a hydrophobic protein containing a significant proportion of alpha helixes. In
this study, a three-dimensional structures of NHX antiporter predicted by in silico 3D homology modeling studies.
The resulting model was refined by energy minimization, subjected to the quality assessment from both geometric
and energetic aspects and was found to be of reasonable quality. Validation of 3D structure was done by plotting
Ramachandran plot and calculation of QMEAN score. This predicted information will help in better understanding
of mechanisms underlying to salt tolerance in monocot plants and use of this information in protein engineering to
improve plants to high salinity conditions.



Cite WeNMR/WestLife

Usage of the WeNMR/WestLife portals should be acknowledged in any publication:
"The FP7 WeNMR (project# 261572), H2020 West-Life (project# 675858) and the EOSC-hub (project# 777536) European e-Infrastructure projects are acknowledged for the use of their web portals, which make use of the EGI infrastructure with the dedicated support of CESNET-MetaCloud, INFN-PADOVA, NCG-INGRID-PT, TW-NCHC, SURFsara and NIKHEF, and the additional support of the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands, Poland, Portugal, Spain, UK, Taiwan and the US Open Science Grid."
And the following article describing the WeNMR portals should be cited:
Wassenaar et al. (2012). WeNMR: Structural Biology on the Grid.J. Grid. Comp., 10:743-767.


The WeNMR Virtual Research Community has been the first to be officially recognized by the EGI.

European Union

WeNMR is an e-Infrastructure project funded under the 7th framework of the EU. Contract no. 261572

WestLife, the follow up project of WeNMR is a Virtual Research Environment e-Infrastructure project funded under Horizon 2020. Contract no. 675858